What is the Difference Between SDS Page and Native Page?
🆚 Go to Comparative Table 🆚The main difference between SDS-PAGE and Native PAGE lies in the type of gel used and the factors that determine protein migration. Here are the key differences:
- Gel Type: SDS-PAGE uses a denaturing gel, while Native PAGE uses a non-denaturing gel.
- Migration Factors: In Native PAGE, protein migration depends on both the mass and structure, whereas in SDS-PAGE, migration is determined only by the protein's mass.
- Denaturation: Protein samples are prepared in a non-denaturing and non-reducing buffer in Native PAGE, while SDS-PAGE involves adding an excessive amount of SDS to coat proteins in a negative charge, covering all the intrinsic charges.
- Protein Stability and Recovery: In SDS-PAGE, proteins are not stable, and recovery is not possible as they are denatured. In contrast, proteins are stable in Native PAGE, and recovery is possible.
In summary, SDS-PAGE separates proteins based on their mass, using a denaturing gel, while Native PAGE separates proteins based on their size, charge, and shape, using a non-denaturing gel.
Comparative Table: SDS Page vs Native Page
Here is a table comparing the differences between SDS-PAGE and Native PAGE:
Feature | SDS-PAGE | Native PAGE |
---|---|---|
Nature of Gel | Denatured, with sodium dodecyl sulfate (SDS) added | Non-denatured |
Denaturation | SDS is added to impart a negative charge on protein samples | No denaturation required |
Basis of Separation | Proteins are separated based on their mass | Proteins are separated based on size and charge, and sometimes shape |
Protein Stability and Recovery | Proteins are not stable and may not be recovered after separation | Proteins remain stable and can be recovered after separation |
In summary, SDS-PAGE is a technique used to separate proteins based on their mass, using a denaturing gel and sodium dodecyl sulfate to impart a negative charge on protein samples. In contrast, Native PAGE is a technique that uses non-denatured gels to separate proteins based on their size, charge, and sometimes shape. Proteins remain stable in Native PAGE and can be recovered after separation.
- SDS Page vs Western Blot
- Gel Electrophoresis vs SDS Page
- Static vs Dynamic Web Pages
- Paging vs Segmentation
- Paging vs Swapping
- Native vs Indigenous
- Native vs Denaturing Gel Electrophoresis
- Webpage vs Website
- Yellow vs White Pages
- Endemic vs Native
- Physical vs Virtual Memory
- Static RAM vs Dynamic RAM
- Static vs Dynamic Memory Allocation
- SDP vs RDP
- Microsoft FrontPage vs Adobe Dreamweaver
- SSD vs HDD
- API vs SDK
- JSP vs Servlets
- PDF vs XPS